The men’s tennis number one was scheduled to play in the Australian Open, which begins on Monday and for which he is the number one seed.
The decision is the latest dramatic twist in the saga over over anti-vaxxer Djokovic’s jab status, which saw his visa revoked when first landed Down Under.
He was first sent to a detention hotel to face deportation before a judge set him free, only for Aussie Immigration Minister Alex Hawke to now use his powers revoke his visa.
But there is likely to be further drama and the door remains open for him to play in the tournament.
His representatives have already said they would launch an immediate appeal in court against any attempt to deport him.